NFATC1 and infection: Next, to determine whether NFATc1 is involved in PD-1 repression during L. donovani infection, nuclear localization of NFATc1 was assessed in infected macrophages, which documented a steady level upto 2 h and started declining only at 4 h post infection (68.9% decrease compared to 2 h infected control, P<0.001; Figure 6d).