UBR5 and colorectal cancer: Given that stabilized β-catenin promotes the E3 ligase activity of UBR5 toward TLE3, we asked whether we could detect UBR5-dependent Ub-TLE3 in the colorectal cancer cell line HCT116, whose Wnt pathway activity is elevated due to a Δ45 mutation in one of its β-catenin alleles (Morin et al., 1997).