For the flies expressing hTDP-43 M337V, however, there was a global enrichment of phospho-TDP-43 signal and a higher incidence of neurons containing ubiquitin-positive puncta within the nuclei and cytoplasm (Fig 5C, 5D & 5E), similar to pathological reports in FTLD and ALS cases [3, 53]. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.