PSEN1 and Alzheimer disease: Minocycline 50 nM, pre-incubated during 15 min, had no effect on either the proportion of active cells or the frequency of astrocytic Ca2+ events within APP/PS1–21 mice (n = 6 slices from 4 animals; p = 0.88 and 0.82 respectively; Additional file 5c) testifying that microglia activation was not involved in the astrocytic hyperactivity setting up at the beginning of Aβ secretion in this AD mouse model.