As expected, in this study, there were vacuolar changes in sertoli cells observed under TEM and downregulated expression of TJs-associated proteins such as various members of Cldn family and Ocln at RNA and protein levels in testis after ZIKV infection, indicating that there might be a decreased synthesis of TJs-associated proteins caused by damaged sertoli cells, which closely linked to reduced integrity of TJs seen after ZIKV infection. The gene discussed is OCLN; the disease is Zika virus infectious disease.