Our findings demonstrated that wogonin inhibited amyloid precursor protein (APP) processing in the β-secretase pathway, the phosphorylation of Tau protein, and Aβ aggregation in human neuroblastoma cells and exhibited protective effects on learning and memory impairments in the Morris water maze test, Y-maze, and the novel object recognition test of 3xTg-AD mice. Here, APP is linked to Alzheimer disease.