According to the results of HMGB3 expression level in CRC cell lines (Fig 2A), we choose SW480 and HCT116 which have lower expression level to establish stable cell lines over-express HMGB3, SW620 and HT29 which have higher expression level to establish stable cell lines inhibiting HMGB3 expression (Fig 2B). The gene discussed is HMGB3; the disease is colorectal carcinoma.