Interestingly, while blocking monocyte recruitment by using a CCR2-neutralizing antibody (MC21) inhibited DSS-induced colitis (Zigmond et al., 2012), in a preliminary experiment we were unable to detect significant differences in the colonic expression of inflammatory genes including Il12b, Ifng, Tnf, and Il1b between isotype and MC21 antibody-treated 3 week old Il10rb-/- mice despite markedly decreased infiltration of Ly6C+ cells (data not shown). The gene discussed is TNF; the disease is colitis.