Quantification of surviving motor neurons from four replicate experiments showed that while depletion of SRSF1 is not detrimental to control co-cultures, motor neuron death was prevented by depletion of SRSF1 in co-cultures derived from three separate C9ORF72-ALS cases (Fig. 3d). The gene discussed is SRSF1; the disease is amyotrophic lateral sclerosis.