The threshold for mutation load in polypeptide-coding genes can be similarly broad, with low levels of mutation causing one type of clinical presentation and higher levels causing another, e.g., m.8993T→G mutation in the ATP synthase 6 (ATP6) gene: at mutation loads above 90%, manifests as maternally inherited Leigh’s syndrome (MILS), at mutation loads in the range of 70–90%, manifests with neuropathy, ataxia and retinitis pigmentosa (NARP), and by contrast, patients with 70% mutation in a tRNA will rarely display overt disease [48]. This evidence concerns the gene MTATP6P1 and maternally-inherited Leigh syndrome.