Himms-Hagen et al. demonstrated that chronic treatment with a β3-AR agonist, CL 316,243, led to the appearance of multilocular brown adipocytes in WAT, promoted thermogenesis, and delayed the development of obesity in rats fed a high-fat diet (Himms-Hagen et al. 1994). The gene discussed is ADRB3; the disease is obesity due to melanocortin 4 receptor deficiency.