When SIRT1-Flag tagged expression plasmid was transfected into Human colon cancer HCT116 cell line, the acetylation levels of SMARCA5, MTA2, HMGA1, EGFR, CHD4 and GOT2 decreased as equal amounts of the proteins were immunoprecipitated (Fig. 6). The gene discussed is CHD4; the disease is malignant colon neoplasm.