As measurement by immunoblotting, UBE2O had no effect on PARP cleavage in NCI-H929 or KMS12 that lack c-Maf (Fig. 6a) but induced PARP cleavage in RPMI-8226 and LP1 that express c-Maf (Fig. 7a), indicating UBE2O probably induced MM cell apoptosis in association with c-Maf expression. This evidence concerns the gene UBE2O and Miyoshi myopathy.