BAY60-2770-induced PKG activation was confirmed using an ELISA based PKG activity assay and Western blot analysis with phosphor-specific VASP at ser239, a substrate of PKG in the ex vivo global ischemia-reperfusion model, demonstrating that BAY60-2770 had a direct effect on cardiac tissue and myocytes. The gene discussed is PRKG1; the disease is ischemia.