No difference was found between ICC numbers in either the distal (34±3; n=3; P=0.0743, Student’s t-test) and proximal colon (81±4; n=3; P=0.4504, Student’s t-test) in sham-operated nNOS−/− compared to control nNOS−/− mice (Fig. 6e,f). Here, NOS1 is linked to intrahepatic cholangiocarcinoma.