RHOA and major depressive disorder: For examples, gastrodin, the main constituent of Gastrodiae Rhizoma, down-regulated the expression of the neuronal cytoskeleton remodeling-related negative regulators Slit1 and RhoA in the hippocampus of rat model of depression [60], San-Huang-Xie-Xin-Tang containing Rhei Radix et Rhizoma attenuated ROCK-II protein expression in U46619-induced primary pulmonary smooth muscle cells [61]; additionally, Leonuri Herba, Scorpio and Gastrodiae Rhizoma were shown have neuroprotective and neuroregenerative effect in vivo and in vitro [62–65].