Tumor cell suspensions were examined by western analysis to confirm reduction of Rictor protein levels in RictorFL/FLNIC samples (Fig. 1c), although some Rictor expression remained, perhaps due to incomplete recombination at floxed Rictor alleles, or to an abundance of non-tumor cells in the tumor suspensions that would not be subjected to Cre-mediated recombination. This evidence concerns the gene RICTOR and neoplasm.