Three out of six mutants (p.E64D, p.F91S, and p.C99W) affected interactions of CIB2 with TMC1 (Fig. 8b, bottom, Supplementary Fig. 10), suggesting that these mutations disrupt the putative TMC1-CIB2 complex in hair cells and thus cause deafness in humans. This evidence concerns the gene CIB2 and deafness.