TP53 and breast neoplasm: DNA was extracted from formalin-fixed, paraffin-embedded samples of the breast tumor, the ovarian tumor, and normal breast tissue using a NucleoSpin DNA FFPE XS system (Takara Bio Inc., Japan), and exons 5–8 of the p53 were amplified by a polymerase chain reaction (98, 56, and 72 °C for 10, 30, and 60 s, respectively) in a Premix Ex Taq® Hot Start Version system (Takara Bio Inc.)using specific primers (Table 1) that were designed based on the data of previous studies [5].