In summary, in this manuscript we have performed a comparative gene expression analysis of human lung epithelial cells expressing active mutant AKT1 (AKT1-E17K), active mutant PI3KCA (PIK3CA-E545K) or that are silenced for PTEN and have identified and validated a set of genes that may represent downstream targets of constitutive PI3K/AKT signalling in lung cancer cells. Here, PIK3CA is linked to lung cancer.