The effect of SIRT6 was further analyzed by injection of a vector expressing SIRT6 lentivirus plasmids into mice before TAC, which resulted in the effective overexpression of SIRT6 mRNA and protein in TAC and sham-operated mice, as indicated by qRT-PCR and western blot analysis (Figures 2A,B). This evidence concerns the gene SIRT6 and persistent truncus arteriosus.