TNF and persistent truncus arteriosus: In addition, assessment of the levels of TNF-α, IL-1β, and IL-10 by ELISA in the heart homogenate of control and pSIRT6-injected mice after TAC or sham surgery showed that SIRT6 significantly attenuated the TAC-induced upregulation of TNF-α and IL-1β and the downregulation of IL-10 (Figure 4E), suggesting that the effect of SIRT6 was mediated by the modulation of inflammatory factors.