Breast cancer Met-1 cells were transducted with a DACH1 expression vector resulting in an ∼4.5-fold increase in DACH1 expression (Fig. 3a) and subsequent reduction of CSC markers CD44, KLF4 and MYC as well as EMT markers including FN1 and VIM (Fig. 3b) by mRNA analysis. This evidence concerns the gene VIM and breast cancer.