As shown in Fig 4b, a significant increase in the level of TPL-2 protein was also detected by immunoblot at 24 hours following BCG infection in AAMs (median relative density pre-infection of 1.68 vs.5.71 post-infection, p = 0.03) but not in CAMs (median relative density pre-infection of 2.5 vs.3 post-infection), Finally, to determine the functional relationship between TPL-2 overexpression and IL-10 production by these macrophages, a competitive pharmacologic inhibitor of TPL-2 (C21H14ClFN6), was utilized, which displays significant selectivity for TPL-2 over other related kinases [26]. This evidence concerns the gene MAP3K8 and infection.