Additional ESCC subgrouping based on T-cell infiltration (CD3i status) and PD-L1 status revealed the CD3ilow / PD-L1low subgroup to be significantly associated with reduced OS (p=0.031), DSS (p=0.012) and DFS (p=0.001) compared to the other subgroups. The gene discussed is CD274; the disease is esophageal squamous cell carcinoma.