To investigate whether some of the inflammatory macrophages in the AT could originate from AT-LSK during metabolic disease, chimeric mice were generated by using standard repopulation assays as previously described (Poglio et al., 2012) by injecting 2.103 sorted scAT-LSK mixed with 2.105 congenic BM cells isolated from congenic CD45 variants. This evidence concerns the gene PTPRC and Other metabolic disease.