Furthermore, we separated five kinds of adipose tissues from high fat diet (HFD) and normal chow diet (NCD) fed mice based on Zfp217’s potential role in obesity, and tested the mRNA level of Zfp217 and Pparg2. When compared with the NCD group, the mRNA level of Zfp217 and Pparg2 for the HFD group showed a similar trend in the visceral adipose tissues (which included epididymal, inguinal and perirenal adipose) (Figure 2C), but presented a reverse trend in the brown adipose and subcutaneous adipose (Figure 2C). Here, PPARG is linked to obesity due to melanocortin 4 receptor deficiency.