Notably, western blot, RPPA and TEM analysis of the tumours treated with the lowest dose (25 mg kg−1 per day) of palbociclib showed higher levels of LC3B-II, Atg-7 and lower levels of p62, as well as the presence of double-membrane electron-dense autophagosomes (Fig. 3c,d and Supplementary Fig. 10l,o–q), demonstrating the induction of intact autophagy. This evidence concerns the gene ATG7 and neoplasm.