HGF and neoplasm: We investigated the expression of major pro- and antiangiogenic factors by quantitative real-time PCR and found that human HGF and human FGF1 were significantly upregulated in tumor xenografts comprising hJHDM1D-AS1-expressing PANC-1 cells and those comprising hJHDM1D-AS1-expressing AsPC-1 cells compared with xenografts comprising control cells (Fig. 5D), suggesting that expression of JHDM1D-AS1 increases the expression of angiogenic factors in cancer cells.