The observed disappearance of fluorescent phagocytes during infection with B. cenocepacia K56-2, however, was not simply due to reduced expression levels of the mpx:GFP and mpeg1:mCherry transgenes; labelling of the leukocyte-specific protein L-plastin or with the macrophage-specific dye Neutral Red [26] also revealed drastically reduced phagocyte numbers in infected embryos, although infected macrophages still stained positive for L-plastin (S5E Fig). This evidence concerns the gene LCP1 and infection.