However, in response to growth factor/amino acids stimulation, LARP1 knockdown significantly delayed and attenuated phosphorylation of these proteins in HEK293T cells (Figure 6A and Figure 6—figure supplement 1A), LnCap (prostate cancer cell line) (Figure 6—figure supplement 1B), and HEK293E cells (insulin sensitive) (Figure 6—figure supplement 1C). This evidence concerns the gene INS and Familial prostate cancer.