We previously used the RWV to establish highly differentiated 3-D intestinal organotypic models derived from human epithelial cell lines and demonstrated their ability to predict in vivo infection outcomes and Salmonella pathogenic mechanisms.7, 16–18 While initiated from a single cell type, these epithelial models spontaneously differentiated into multiple epithelial cell types normally found in the intestine, including enterocytes, goblet cells, Paneth cells, and M/M-like cells, while exhibiting extensive tight junction formation, apical-basolateral polarity, and mucin production. Here, MUC5AC is linked to infection.