PRC1 and squamous cell carcinoma: We next performed qRT-PCR and Western blot analysis to determine the expression level of PRC1 in 4 human NSCLC cell lines, which include one squamous carcinoma cell lines and 3 adenocarcinoma cell lines, and HBE We observed that PRC1 expression was elevated in three lung adenocarcinoma cell lines, whereas PRC1 expression was lower in one lung squamous carcinoma cell lines and HBE (Fig. 3a, Additional file 3: Figure S2A).