Occasional neurones, Schwann cells and macrophages (as assessed by positive staining for NeuN, S100 and F480 respectively) appeared ZIKV +ve at 72 hpi following infection with ZIKV at MOI 3.0 (Fig. 6a-d), however the overall levels of infection were barely above background levels, even in Ifnar1 KO cultures. Here, IFNAR1 is linked to infection.