In addition, we found that capsaicin at 100 μM induced autophagy via the activation of the class III PI3K/Beclin-1/Bcl-2 signaling pathway (Figure 3); at 150 μM, capsaicin caused autophagosome formations because of the increase in Atg and LC3-ll levels (Figure 4), and capsaicin at 300 μM triggered NPC-TW01 cell apoptosis due to increases in caspase-3 activity (Figure 5); thereby confirming that capsaicin stimulated NPC cells’ autophagy at lower concentrations. This evidence concerns the gene BECN1 and nasopharyngeal carcinoma.