Thirty days after ZIKV infection we saw a strong polyfunctional CD4+ T-cell response, where the CD4+ T cells from BS14 made IFN-γ (0.70%), TNF-α (9.6%) and CD107a (2%) in response to the E peptide pool stimulation (Fig. 7g–i). This evidence concerns the gene IFNG and Zika virus infectious disease.