Moreover, adenovirus‐mediated transfection of GFP‐LC3 H9c2 cells with sip65 attenuated the increase in the number of GFP‐LC3 dots induced by high glucose (Fig. 8F and G), suggesting that expression of p65 is essential for the up‐regulation of autophagy in diabetes, and that the suppression of p65 has a protective effect against DCM. Here, RELA is linked to diabetes mellitus.