PNPLA3 and fatty liver disease: Neither ablation nor overexpression of wild-type PNPLA3 affects the liver fat content in mice, whereas transgenic mice with hepatic specific overexpression of human 148M or PNPLA3 I148M knock-in mice exhibit increased hepatic TAG contents and LD sizes and develop hepatic steatosis (Basantani et al., 2011, Li et al., 2012, Pirazzi et al., 2012, Smagris et al., 2015).