CCN2 and acute kidney injury: However, mice with IR-induced AKI exhibited enhanced mRNA and protein expression of Col I, TGFβ, CCN2 and CCN3 at 14 days post IR compared with the sham controls (ANOVA, surgery main and interaction effects, F(1,28)=27.44–36.01, P<0.0001; Tukey’s test, P<0.05; Figures 4a–c).