CDCA8 and infection: Maggio et al. have compared adenoviral vectors (co-infection of Cas9 and sgRNA expressing viruses) in multiple cell lines and showed 31% and 18% target gene (the AAVS1 locus) knockout frequency for HeLa cells and U-2 OS cells respectively [11], compared to ± 60% and 65% as observed by us using baculoviral delivery of Cas9 and targeting the CDCA8 locus (Fig 2C for U-2 OS cells and Fig 3B for HeLa cells).