The absence of mitosis in the surviving MCF7GFP-TRPV1 cells subsequently didn’t allow for the establishment of stable MCF7GFP-TRPV1 clones, although we had been successful to establish cell clones permanently expressing ectopic TRPV1 protein using non-tumor derived cell lines such as HaCaT, a spontaneously immortalized keratinocyte cell line from adult human skin [33], or NIH-3T3 cells, a spontaneously immortalized mouse embryo fibroblast cell line [49]. Here, TRPV1 is linked to neoplasm.