To further link PKC-η and MARCKS function to bacterial survival within host cells, we demonstrated that PKC-η or MARCKS depletion via siRNA resulted in a ~10-fold and ~2.5-fold reduction, respectively, in the number of viable intracellular B. thailandensis in A549 cells following infection for 24 h, compared to cells treated with non-targeting siRNA (siR-CTL) (Figure 4A, light gray bars). This evidence concerns the gene MARCKS and infection.