CASP3 and melanoma: Our next studies used the IC50 dose of ASA at 72 hour time point and demonstrated that ASA-mediated inhibition of these melanoma cell survival was due to the induction of apoptosis as measured by immunoblotting for cleaved caspase 3, luminescence and fluorescence-based caspase-3/7 activity (Figure 1D-1E and Supplementary Figure 1).