TP53 and osteosarcoma: Both T4 (within the PLGA group) and T6 (within the PLGA-PEG group) showed the highest knockdown efficiency to silence mutant p53, however, T4 (10% PLGA) between T4 and T6 stands out as the nanoparticle with the least cytotoxicity and best suited for siRNA delivery into the osteosarcoma cell line.