In order to further verify the abovementioned research results, we randomly selected 50 specimens and quantitatively determined the content of PIWIL1 mRNA in the cancer tissue and adjacent tissue, the results of which suggested that the content of PIWIL1 mRNA in cancer tissue was markedly higher than that in the adjacent tissues (P < 0.001, Figure 2). This evidence concerns the gene PIWIL1 and cancer.