Masson’s trichrome staining showed that collagen depositions were significantly increased both in TLR4 wild (Fig. 1B and C, P < 0.01) and TLR4 mutation mice (Fig. 1B and C, P < 0.05) on day 28 post-infection (p.i.), compared with no-infected mice. This evidence concerns the gene TLR4 and infection.