For example, it was shown that TLR4 signaling pathway promotes the activations of HSCs and contributes to liver fibrosis through coordination of TGF-β signaling in the liver fibrosis of no-infectious disease22, 34 while TLR4 as a negative regulator prevents fibrosis in the models of lung injuries35, 36. This evidence concerns the gene TGFB1 and Hepatic fibrosis.