Combining methods of efficient generation of antigen-specific antitumor T cell populations and subsequent isolation of activated cytotoxic T cells appropriate for clinical use, this approach can be used to generate functionally active antigen-specific T lymphocytes from peripheral blood mononuclear cells of patients with HER2-positive malignancies for adoptive T-cell transfer to eliminate HER2-positive tumor cells, prevent metastasis and relapse. Here, ERBB2 is linked to neoplasm.