As shown in Fig. 1C, AZD6244 induced Akt Ser473 phosphorylation only in HER2‐positive SkBr3 and BT474 but not in HER2‐negative MDA‐MB‐468, MDA‐MB‐231, and MCF‐7 breast cancer cell lines. This evidence concerns the gene ERBB2 and breast carcinoma.