With the loss of methylation of hsa‐let‐7 g, there were increases in the expression of the CCND1, SMOX, ADM and CDKN1A genes, and a decrease in that of the VSNL1 gene, which are in accordance with the results in the NTD cases; in contrast, EGR1, SCPEP, DNMT3B, FBXW7 and PGMIL1 genes showed contradictory results compared with the NTD samples. This evidence concerns the gene CDKN1A and neural tube defect.