Based on the above results indicating that the administration of ECD and LGZGD can suppress the development of HFD-induced fatty liver, we presume that inhibition of TNF-α, NF-κB, and IRS-1Ser307 phosphorylation expressions might be major contributor to the beneficial effects of ECD and LGZGD on decreasing hepatic lipid accumulation caused by IR. This evidence concerns the gene TNF and fatty liver disease.