Repeated injections of E2HSA in KKAy mice showed similar anti-diabetic benefits like that in db/db mice, including attenuating variations in fasting and non-fasting blood glucose levels, correcting the abnormal homeostasis of insulin and glucagon, and improving β-cell function, as well as ameliorating dyslipidemia, decreasing food intake and body weight, which further demonstrated the effectiveness of E2HSA. Here, INS is linked to metabolic syndrome.